多克隆抗體制備（抗原為重組蛋白）
關于多克隆抗體制備，我司可定制兔來源、小鼠來源以及羊來源的多抗，詳情請見：
| 項目 | 明細 |
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必要信息（客戶提供）
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1. Full name of the gene, NCBI # or Uniprot ID. 2. If possible, provide us the full sequence of the gene (optional). 3. Expected application: ELISA? WB? IHC? ICC? IF? Flow Cyto?..... 4. What sample the antibody plan to detect? Please indicated this in detail, e.g. A431 cell lysate, mouse lung tissue, etc. 5. Other requirements: like need tag at the sequence; just need anti-serum? Manufacture according to the customer’ sequence, etc. |
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制備流程
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1. Analyze the sequence, design and synthesize the gene with tags. (If for more than 300 genes, the price will be increased). 2. Construct the carrier and express the protein in E.Coli. 3. Nickel column and Ionic column purification. 4. Immunize rabbits (usually at least 4 rabbits), mouse, or goat with this protein (Multiple immunization for each rabbit, mouse or goat), during this period, we need collect the sample and test to determined the immunization. 1. Collect the anti-serum after immunization and test. 6. Purify the antibody from the anti-serum if the results are good (if the testing results are not good, we will immunize again). |
| 質量標準 | ELISA (1:20000-100000) with high stability & sensitivity |
| 周期 | 80 days (maybe prolonged or decreased for different antibodies) |
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提供的成品
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1. Purified antibody, 10-50mg. (For goat polyclonal, will be greater than 100mg) 2. All the rest anti-serum (End user can use this for testing or purifying the antibody) or the ascites for mouse polyclonal, 10ml. (For goat polyclonal, will be greater than 100ml) 3. Rest immunogen, the recombinant protein, 0.5-1mg. 4. Report for the whole service & manual |
| 備注 |
1. If customer can provide the genes (requirements: volume: 3-5mg, purity > 85%), price will be cheaper. 2. If customer just need anti-serum, not the purified antibodies, the price will be different, please inquiry and indicate at the beginning. 3. We respect the intellectual property of the antibody, will not sell it commercially unless get the permit from customer. |
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