產品名稱：細胞膜蛋白與細胞漿蛋白提取試劑盒
- 產品編號
- BWR1007
- 產品規格
- 100次
- 價格
- 878.00
- 來源
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- 交叉反應
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- 免 疫 原
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- 產品簡介
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- Membrane and Cytosol Protein Extraction Kit provides a simple and convenient way for extracting membrane and cytosol protein from cultured cells and tissues. This kit can extract not only the membrane protein on the plasma membrane, also the membrane protein on the Mitochondrial, Endoplasmic reticulum and Golgiapparatus.
- This kit breaks cells properly by homogenizing, and through low speed centrifugation, discards nucleus and precipitate produced by the unbroken cells. Then, collect the supernatant and centrifuge at high speed to obtain the cell membrane pellets and the cytosol protein included supernatant. At last, extract the membrane protein from the pellets through Membrane Protein Extraction Reagent.
- It takes about 90 min to complete separating and extracting the membrane and cytosol protein from cultured cells and tissues. Extracted proteins can be used for SDS-PAGE, Western, enzyme activity and other subsequent experiment.
- The Membrane Protein Extraction Reagent contains protease and phosphatases inhibitor, EDTA, etc. Thus, it is not suitable for determining the activities of enzymes which affected by these inhibitors (like protease, phosphatases), but the extracted membrane or cytosol proteins can be used for detection of protein phosphorylation levels.
- The kit can be used for 100 samples. User can adjust the volume of reagent according to different sample volumes.
Components Size Storage Instruction Membrane Protein Extraction Reagent A (MER A) 100 ml Store at 4℃ for one year. Membrane Protein Extraction Reagent B (MER B) 30 ml Store at 4℃ for one year.
- 產品別名
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- 產品成分
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- 檢測范圍
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- 檢測樣品
- -
- 產品應用
- WB
- 保存條件
- -20℃一年
- 背景資料
- -
- 參考資料
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