產品名稱：Monoclonal Anti- β-Tubulin antibody
- 產品編號
- BTL1037
- 產品規格
- 100ug
- 價格
- 558 .00
- 來源
- Mouse
- 交叉反應
- Human, mouse, rat, monkey, Chinese hamster, dog
- 免 疫 原
- Protein fragment on C-terminal of human β-Tubulin.
- 產品簡介
- Isotype IgG1
Host Mouse
Reactivity Human, mouse, rat, monkey, Chinese hamster, dog
Specificity Detect the endogenous levels of total β-Tubulin protein.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- -
- 檢測樣品
- -
- 產品應用
- WB(1:5000) IF(1:1000) ELISA(1:1000-2000)
- 保存條件
- -20°C一年，避免反復凍融。
- 背景資料
- Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8.1 Mutant huntingtin, disrupted intracellular transport and insulin secretion by direct interference with microtubular beta-tubulin. Mutant huntingtin impaired glucose-stimulated insulin secretion in insulin-producing beta cells, without altering stored levels of insulin. Smith et al. (2009) proposed a novel pathogenetic process by which mutant huntingtin may disrupt hormone exocytosis from beta cells and possibly impair vesicular transport in any cell that expresses the pathogenic protein.2
- 參考資料
- 1. Williams RC Jr, Shah C, Sackett D (November 1999). "Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels". Anal Biochem 275 (2): 265–7. 2. Smith, R., Bacos, K., Fedele, V., Soulet, D., Walz, H. A., Obermuller, S., Lindqvist, A., Bjorkqvist, M., Klein, P., Onnerfjord, P., Brundin, P., Mulder, H., Li, J.-Y. Mutant huntingtin interacts with beta-tubulin and disrupts vesicular transport and insulin secretion. Hum. Molec. Genet. 18: 3942-3954, 2009.
