產品名稱：beta-actin antibody
- 產品編號
- BTL1026
- 產品規格
- 100ug
- 價格
- 680.00
- 來源
- Rabbit
- 交叉反應
- Human, mouse, rat, monkey, chicken, pig, cow, rabbit, D. Melanogaster
- 免 疫 原
- A synthetic peptide (conjugated to KLH) corresponding to N-terminal residues of human β-Actin.
- 產品簡介
- Isotype IgG
Host Rabbit
Reactivity Human, mouse, rat, monkey, chicken, pig, cow, rabbit, D. Melanogaster
Specificity Detect endogenous level of total β-Actin protein.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- -
- 檢測樣品
- -
- 產品應用
- WB(1:1500-2000) IHC-P(1:100-1000) ELISA (1:1000-5000)
- 保存條件
- -20°C一年,避免反復凍融。
- 背景資料
- Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.1 Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.2 Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.
- 參考資料
- 1. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 2. Ji, Y., Ferracci, G., Warley, A., Ward, M., Leung, K.-Y., Samsuddin, S., Leveque, C., Queen, L., Reebye, V., Pal, P., Gkaliagkousi, E., Seager, M., Ferro, A. Beta-actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc. Nat. Acad. Sci. 104: 8839-8844, 2007.
