產品名稱：Monoclonal Anti-MBP Tag antibody
- 產品編號
- BTL1014
- 產品規格
- 100ug
- 價格
- 558 .00
- 來源
- Mouse
- 交叉反應
- All
- 免 疫 原
- Recombinant MBP protein with full length
- 產品簡介
- Isotype IgG1
Host Mouse
Reactivity All
Product Form Liquid (1mg/ml)
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- -
- 檢測樣品
- -
- 產品應用
- WB(1:1000-10000)
- 保存條件
- -4°C 一年
- 背景資料
- Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
- 參考資料
- 1.Claude V. Maina, Paul D. Riggs, Andres G. Grandea III, Barton E. Slatko, Laurie S. Moran, John A. Tagliamonte, Larry A. McReynolds, di Guan Chu. "An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein" Gene Volume 74, Issue 2, 30 December 1988, Pages 365–373
