產品名稱：Monoclonal Anti-His Tag antibody
- 產品編號
- BTL1010
- 產品規格
- 100ug
- 價格
- 558 .00
- 來源
- Mouse
- 交叉反應
- All
- 免 疫 原
- A synthetic peptide 6 × His conjugated to KLH.
- 產品簡介
- Isotype IgG1
Host Mouse
Reactivity All
SpecificityDetect over-expressed or recombinant proteins containing the 6 × His epitope tag. - 產品別名
- -
- 產品成分
- -
- 檢測范圍
- -
- 檢測樣品
- -
- 產品應用
- WB(1:5000) IP(1:100) IF(1:2000) ELISA(1:2000)
- 保存條件
- -20°C一年,避免反復凍融。
- 背景資料
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.
- 參考資料
- 1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285–6. 2. Gavin, AC; B?sche, M; Krause, R; Grandi, P; Marzioch, M; Bauer, A; Schultz, J; Rick, JM et al. (2002). "Functional organization of the yeast proteome by systematic analysis of protein complexes". Nature 415 (6868): 141–7.
