產品名稱：Monoclonal Anti-HA Tag antibody
- 產品編號
- BTL1009
- 產品規格
- 100ug
- 價格
- 558 .00
- 來源
- Mouse
- 交叉反應
- All
- 免 疫 原
- A synthetic peptide YPYDVPDYA conjugated to KLH.
- 產品簡介
- Isotype IgG1
Host Mouse
Reactivity All
SpecificityDetect over-expressed proteins containing the HA epitope tag. - 產品別名
- -
- 產品成分
- -
- 檢測范圍
- -
- 檢測樣品
- -
- 產品應用
- WB(1:5000-10000) IP(1:100-500) IF(1:1000) ELISA(1:2000)
- 保存條件
- -20°C一年,避免反復凍融。
- 背景資料
- Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. The vectors have been engineered so that the GST carrier can be cleaved from fusion proteins by digestion with site-specific proteases such as thrombin or blood coagulation factor Xa, following which, the carrier and any uncleaved fusion protein can be removed by absorption on glutathione-agarose. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- 參考資料
- 1. Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. 1988, 67(1):31-40. PMID:3047011
