
產品名稱:C-Peptide Mouse ELISA
- 產品編號
- BEK1266
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
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- 交叉反應
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- 免 疫 原
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- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- C-Peptide antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- C-Peptide antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C-Peptide amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C-Peptide can be calculated.
- 產品別名
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- 產品成分
- -
- 檢測范圍
- 1 ng/ml-40 ng/ml
- 檢測樣品
- -
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Connecting peptide, or C-peptide, is a short 31-amino-acid protein that connects insulin's A-chain to its B-chain in the proinsulin molecule. C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. It has been found to exert beneficial effects in many tissues affected by diabetic microvascular complications, including the kidneys. C-peptide at physiological concentrations effectively reduces diabetes-induced glomerular hyperfiltration via constriction of the afferent arteriole, dilation of the efferent arteriole, and inhibition of tubular reabsorption in experimental models of type 1 diabetes.
- 參考資料
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