產品名稱：HFABP, FABP3 Human ELISA Kit
- 產品編號
- BEK1254
- 產品規格
- 96T
- 價格
- 2400.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HFABP monoclonal antibody was pre-coated onto 96-well plates. The standards and test samples were added to the wells and any HFABP present is bound by the immobilized antibody. After washing
away any unbound substances, the HRP conjugated anti-HFABP monoclonal antibody was added to wells as detection antibodies. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding stop solution. The density of yellow is proportional to the HFABP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HFABP can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-human HFABP antibody
2. Lyophilized human HFABP standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30 ml
4. HRP conjugated anti-human HFABP antibody (Concentrated): 130 μl. Dilution: 1:100 (Store at 4℃, do NOT store it at -20℃)
5. Antibody diluent buffer: 12 ml
6. TMB substrate: 10 ml
7. Stop solution: 10 ml
8. Wash buffer: 30 ml (25x). Dilution: 1:25
Note: Reconstitute standards and test samples with Kit Component 3.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 15.6?pg/ml - 1000 pg/ml
- 檢測樣品
- For quantitative detection of human HFABP?in?serum, cell culture supernatants?or tissue homogenate.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The serum HFABP level is an effective biomarker for detecting AKI in non-surgical intensive care patients. HFABP expression is significantly higher in in-stent restenosis, ISR tissue than in non-ISR tissue from diabetic and nondiabetic minipigs. Overexpression of HFABP induces multiple pathway-related promotion of inflammation, growth and migration in vascular SMCs, suggesting a potential role in coronary artery ISR. hFABP is a rapid marker of perioperative myocardial damage and peaks earlier than CKMB or TnI. The kinetics of marker proteins in serial samples immediately after reperfusion is more suitable for the detection of perioperative myocardial infarction than a fixed cut-off level.
- 參考資料
- 1. Shirakabe A, Kobayashi N, Hata N, Shinada T, Tomita K, Tsurumi M, Okazaki H, Matsushita M, Yamamoto Y, Yokoyama S, Asai K, Shimizu W. The serum heart-type fatty acid-binding protein (HFABP) levels can be used to detect the presence of acute kidney injury on admission in patients admitted to the non-surgical intensive care unit. BMC Cardiovasc Disord. 2016 Sep 5;16(1):174. 2. Chen K, Chen QJ, Wang LJ, Liu ZH, Zhang Q, Yang K, Wang HB, Yan XX, Zhu ZB, Du R, Zhang RY, Shen WF, Lu L. Increment of HFABP Level in Coronary Artery In-Stent Restenosis Segments in Diabetic and Nondiabetic Minipigs: HFABP Overexpression Promotes Multiple Pathway-Related Inflammation, Growth and Migration in Human Vascular Smooth Muscle Cells. J Vasc Res. 2016;53(1-2):27-38. Epub 2016 Jul 2. 3. Petzold T, Feindt P, Sunderdiek U, Boeken U, Fischer Y, Gams E. Heart-type fatty acid binding protein (hFABP) in the diagnosis of myocardial damage in coronary artery bypass grafting. Eur J Cardiothorac Surg. 2001 Jun;19(6):859-64.
