產品名稱：Insulin Rat ELISA Kit
- 產品編號
- BEK1243
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Rat
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Insulin monoclonal antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-Insulin polyclonal antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Insulin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Insulin can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-Rat Insulin antibody
2. Lyophilized insulin standards: 6 tubes (0 uIU/ml, 8 uIU/ml, 16 uIU/ml, 32 uIU/ml, 80 uIU/ml, 140 uIU/ml) (Please check the protocol for standard preparation)
3. HRP conjugated anti-Rat Insulin antibody (RTU): 6 ml
4. TMB substrate A: 7 ml
5. TMB substrate B: 7 ml
6. Stop solution: 7 ml
7. Wash buffer (25X): 30ml
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 0 uIU/ml-140 uIU/ml
- 檢測樣品
- ?For quantitative detection of Insulin in Rat serum, plasma, body fluids, tissue lysates or cell culture supernates.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues, and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.
- 參考資料
- 1. Steiner, D. F., Oyer, P. E. The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc. Nat. Acad. Sci. 57: 473-480, 1967. 2. Dandona, P., Aljada, A., Mohanty, P., Ghanim, H., Hamouda, W., Assian, E., Ahmad, S. Insulin inhibits intranuclear nuclear factor kappa-B and stimulates I-kappa-B in mononuclear cells in obese subjects: evidence for an anti-inflammatory effect? J. Clin. Endocr. Metab. 86: 3257-3265, 2001. 3. Frosig, C., Rose, A. J., Treebak, J. T., Kiens, B., Richter, E. A., Wojtaszewski, J. F. P. Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160. Diabetes 56: 2093-2102, 2007.
