產品名稱：SDF-1 Rat ELISA Kit
- 產品編號
- BEK1241
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Rat
- 免 疫 原
- -
- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-SDF-1 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-SDF-1 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SDF-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SDF-1 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-rat SDF-1 antibody
2. Standard: 0.5 ml (2250 pg/ml)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-rat SDF-1 antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 70 pg/ml-2000pg/ml
- 檢測樣品
- ?For quantitative detection of SDF-1 in rat serum, plasma, cell culture supernatant or tissue samples.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Stromal cell-derived factor-1 (SDF-1) is small cytokine belonging to the intercrine family, member of which activate leukocytes and is often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The mouse SDFs are expressed in all cell lines examined except blood. SDF-1 is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene. Bleul et al. (1996) found that SDF1 is a highly efficacious lymphocyte chemoattractant. In addition, SDF1 induces intracellular actin polymerization in lymphocytes. By blocking CXCR4, a major co-receptor for HIV-1 entry, CXCL12 acts as an endogenous inhibitor of CXCR4-tropic HIV-1 strains.
- 參考資料
- 1. De La Luz Sierra M, Yang F, Narazaki M, Salvucci O, Davis D, Yarchoan R, Zhang HH, Fales H, Tosato G (April 2004). "Differential processing of stromal-derived factor-1alpha and stromal-derived factor-1beta explains functional diversity". Blood 103 (7): 2452–9. 2. Bleul, C. C., Fuhlbrigge, R. C., Casasnovas, J. M., Aiuti, A., Springer, T. A. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. Exp. Med. 184: 1101-1109, 1996. 3. Oberlin E, Amara A, Bachelerie F, Bessia C, Virelizier JL, Arenzana-Seisdedos F, Schwartz O, Heard JM, Clark-Lewis I, Legler DF, Loetscher M, Baggiolini M, Moser B. (August 1996). "The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1.". Nature 382 (6594): 833–5.
