產品名稱：VEGF-R3 Human ELISA Kit
- 產品編號
- BEK1234
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-VEGFR3 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-VEGFR3 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were
added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGFR3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGFR3 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-human VEGFR3 antibody
2. Standard: 0.5ml (900 pg /mL)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-human VEGFR3 antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 30 pg/ml-850 pg/ml
- 檢測樣品
- For quantitative detection of VEGFR3 in Human serum, plasma, urine, cell culture supernatant or tissue samples.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- VEGF receptors are receptors for vascular endothelial growth factor (VEGF). They play an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis.There are three main subtypes of VEGFR, numbered 1, 2 and 3. VEGFR3 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. VEGFR3 provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells.
- 參考資料
- 1. Cursiefen, C., Chen, L., Saint-Geniez, M., Hamrah, P., Jin, Y., Rashid, S., Pytowksi, B., Persaud, K., Wu, Y., Streilein, J. W., Dana, R. Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision. Proc. Nat. Acad. Sci. 103: 11405-11410, 2006. 2. Dumont, D. J., Jussila, L., Taipale, J., Lymboussaki, A., Mustonen, T., Pajusola, K., Breitman, M., Alitalo, K. Cardiovascular failure in mouse embryos deficient in VEGF receptor-3. Science 282: 946-949, 1998.
