產品名稱： Human VEGF-D ELISA Kit
- 產品編號
- BEK1231
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-VEGF-D antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-VEGF-D antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGF-D amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGF-D can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-human VEGF-D antibody
2. Standard: 0.5ml (360pg /mL)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-human VEGF-D antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 10 pg/ml-300 pg/ml
- 檢測樣品
- For quantitative detection of VEGF-D in human serum, plasma, urine, cell culture supernatant or tissue samples.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- C-fos induced growth factor (FIGF) (or vascular endothelial growth factor D, VEGF-D) is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. VEGF-D has been established to be over-expressed in both tumor tissues and patients’ serum samples in several types of human cancer. In addition, VEGF-D expression has been implicated with increased incidence of regional lymph node metastasis. In experimental mice study, genetically modified tumor cell that was forced to produce VEGF-D protein have been established to boost up regional lymph nodes metastases.
- 參考資料
- 1. Achen, M. G., Jeltsch, M., Kukk, E., Makinen, T., Vitali, A., Wilks, A. F., Alitalo, K., Stacker, S. A. Vascular endothelial growth factor D (VEGF-D) is a ligand for the tyrosine kinases VEGF receptor 2 (Flk1) and VEGF receptor 3 (Flt4). Proc. Nat. Acad. Sci. 95: 548-553, 1998. 2. Stacker, S. A., Caesar, C., Baldwin, M. E., Thornton, G. E., Williams, R. A., Prevo, R., Jackson, D. G., Nishikawa, S., Kubo, H., Achen, M. G. VEGF-D promotes the metastatic spread of tumor cells via the lymphatics. Nature Med. 7: 186-191, 2001.
