產品名稱：VEGF-B Human ELISA Kit
- 產品編號
- BEK1229
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-VEGF-B antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-VEGF-B antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then,
unbound conjugates were washed away with
wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGF-B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGF-B can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-human VEGF-B antibody
2. Standard: 0.5ml (360 pg /mL)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-human VEGF-B antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 8 pg/ml-300 pg/ml
- 檢測樣品
- For quantitative detection of VEGF-B in human serum, plasma, urine, cell culture supernatant or tissue samples.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Vascular endothelial growth factor B also known as VEGF-B is a regulator of blood vessel physiology, belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. It signals via the endothelial receptor VEGFR1. VEGF-B has a role in endothelial targeting of lipids to peripheral tissues. It also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.
- 參考資料
- 1. Hagberg, C. E., Falkevall, A., Wang, X., Larsson, E., Huusko, J., Nilsson, I., van Meeteren, L. A., Samen, E., Lu, L., Vanwildemeersch, M., Klar, J., Genove, G., Pietras, K., Stone-Elander, S., Claesson-Welsh, L., Yia-Herttuala, S., Lindahl, P., Eriksson, U. Vascular endothelial growth factor B controls endothelial fatty acid uptake. Nature 464: 917-921, 2010. 2. Li Y, Zhang F, Nagai N, Tang Z, Zhang S, Scotney P, Lennartsson J, Zhu C, Qu Y, Fang C, Hua J, Matsuo O, Fong GH, Ding H, Cao Y, Becker KG, Nash A, Heldin CH, Li X (March 2008). "VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats". J. Clin. Invest. 118 (3): 913–23. 3. Sun Y, Jin K, Childs JT, Xie L, Mao XO, Greenberg DA (October 2004). "Increased severity of cerebral ischemic injury in vascular endothelial growth factor-B-deficient mice". J. Cereb. Blood Flow Metab. 24 (10): 1146–52.
