產品名稱：TIMP-2 Human ELISA Kit
- 產品編號
- BEK1210
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-2 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-Human TIMP-2 antibody
2. Lyophilized Human TIMP-2 standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Human TIMP-2 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer(25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 156 pg/ml-10,000 pg/ml
- 檢測樣品
- For quantitative detection of TIMP-2 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- TIMP metallopeptidase inhibitor 2, also known as TIMP-2, is a 21-kD protein, which is secreted by human melanoma cells and binds to type IV collagenase proenzyme secreted by the same cells. The TIMP2 gene is a member of the TIMP gene family, It is encoded by 5 exons spanning 83 kb of genomic DNA. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. It has an amino acid sequence with many similarities to human tissue inhibitor of metalloproteinase (TIMP1). TIMP2 is a tissue inhibitor of metalloproteinases, TIMP2 induced a decrease in total protein tyrosine phosphatase (PTP) activity associated with beta-1 integrin subunits as well as dissociation of the phosphatase SHP1 from beta-1.
- 參考資料
- 1. Stetler-Stevenson, W. G., Krutzsch, H. C., Liotta, L. A. Tissue inhibitor of metalloproteinase (TIMP-2): a new member of the metalloproteinase inhibitor family. J. Biol. Chem. 264: 17374-17378, 1989. 2. Hammani, K., Blakis, A., Morsette, D., Bowcock, A. M., Schmutte, C., Henriet, P., DeClerck, Y. A. Structure and characterization of the human tissue inhibitor of metalloproteinases-2 gene. J. Biol. Chem. 271: 25498-25505, 1996.
