產品名稱：NT-4 Mouse ELISA Kit
- 產品編號
- BEK1177
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Mouse
- 免 疫 原
- -
- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-NT-4 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-NT-4 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the
wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NT-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NT-4 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-mouse NT-4 antibody
2. Standard: 0.5ml (22500pg /mL)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-mouse NT-4 antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 400 pg/ml-20,000 pg/ml
- 檢測樣品
- For quantitative detection of NT-4 in mouse serum, plasma, urine, cell culture supernatant or tissue samples.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Neurotrophins act as survival and differentiation factors in the nervous system and have been detected in the developing rodent testis. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). Neurotrophin-4 (NT-4), is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. Mammalian NT4 had many unusual features compared to the previously identified neurotrophins and was less conserved evolutionarily than the others; however, mammalian NT4 displayed bioactivity and TRK receptor specificity similar to that of Xenopus NT4.
- 參考資料
- Reference 1. Ibanez, C. F. Neurotrophin-4: the odd one out in the neurotrophin family. Neurochem. Res. 21: 787-793, 1996. 2. Ip, N. Y., Ibanez, C. F., Nye, S. H., McClain, J., Jones, P. F., Gies, D. R., Belluscio, L., Le Beau, M. M., Espinosa, R., III, Squinto, S. P., Persson, H., Yancopoulos, G. D. Mammalian neurotrophin-4: structure, chromosomal localization, tissue distribution, and receptor specificity. Proc. Nat. Acad. Sci. 89: 3060-3064, 1992. 3. Ip, N. Y., Ibanez, C. F., Nye, S. H., McClain, J., Jones, P. F., Gies, D. R., Belluscio, L., Le Beau, M. M., Espinosa, R., III, Squinto, S. P., Persson, H., Yancopoulos, G. D. Mammalian neurotrophin-4: structure, chromosomal localization, tissue distribution, and receptor specificity. Proc. Nat. Acad. Sci. 89: 3060-3064, 1992.
