產品名稱：IL-4 Mouse ELISA Kit
- 產品編號
- BEK1104
- 產品規格
- 96T
- 價格
- 2400.00
- 來源
- -
- 交叉反應
- Mouse
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates
were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-4 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-Mouse IL-4 antibody
2. Lyophilized Mouse IL-4 standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Mouse IL-4 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer(25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 7.8 pg/ml-500 pg/ml
- 檢測樣品
- For quantitative detection of IL-4 in mouse serum, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月, -20℃一年
- 背景資料
- Interleukin-4(IL-4) is an 18-kD glycoprotein, it has 4 exons and is approximately 10 kb. It has a compact, globular fold, stabilised by 3 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation B cells into Plasma Cells, and it is a key regulator in humoral and adaptive immunity. IL-4 also has been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma.
- 參考資料
- 1. Carr C, Aykent S, Kimack NM, Levine AD (February 1991). "Disulfide assignments in recombinant mouse and human interleukin 4". Biochemistry 30 (6): 1515–23. 2. Walter MR, Cook WJ, Zhao BG, Cameron RP, Ealick SE, Walter RL, Reichert P, Nagabhushan TL, Trotta PP, Bugg CE (October 1992). "Crystal structure of recombinant human interleukin-4". J. Biol. Chem. 267 (28): 20371–6. 3. Walter MR, Cook WJ, Zhao BG, Cameron RP, Ealick SE, Walter RL, Reichert P, Nagabhushan TL, Trotta PP, Bugg CE (October 1992). "Crystal structure of recombinant human interleukin-4". J. Biol. Chem. 267 (28): 20371–6. 4. Hosoyama T, Aslam MI, Abraham J, Prajapati SI, Nishijo K, Michalek JE, Zarzabal LA, Nelon LD, Guttridge DC, Rubin BP, Keller C (May 2011). "IL-4 R Drives Dedifferentiation, Mitogenesis, and Metastasis in Rhabdomyosarcoma". Clin Cancer Res 17 (9): 2757–2766.
