產品名稱：IL-3 Human ELISA Kit
- 產品編號
- BEK1101
- 產品規格
- 96T
- 價格
- 2400.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were
used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-3 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-Human IL-3 antibody
2. Lyophilized Human IL-3 standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Human IL-3 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 15.6 pg/ml-1000 pg/ml
- 檢測樣品
- For quantitative detection of IL-3 in human serum, body fluids, tissue lysate or cell culture supernate.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月, -20℃一年
- 背景資料
- Interleukin 3 (IL-3) is a hematopoietic colony-stimulating factor that is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. It can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL3 infusion expands an early cell population that subsequently requires the action of a later-acting factor such as GMCSF to complete its development.
- 參考資料
- 1. Chavany, C., Vicario-Abejon, C., Miller, G., Jendoubi, M. Transgenic mice for interleukin 3 develop motor neuron degeneration associated with autoimmune reaction against spinal cord motor neurons. Proc. Nat. Acad. Sci. 95: 11354-11359, 1998. 2. Donahue, R. E., Seehra, J., Metzger, M., Lefebvre, D., Rock, B., Carbone, S., Nathan, D. G., Garnick, M., Sehgal, P. K., Laston, D., LaVallie, E., McCoy, J., Schendel, P. F., Norton, C., Turner, K., Yang, Y.-C., Clark, S. C. Human IL-3 and GM-CSF act synergistically in stimulating hematopoiesis in primates. Science 241: 1820-1823, 1988. 3. Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell 47 (1): 3–10.
