產品名稱：IL-2 Rat ELISA Kit
- 產品編號
- BEK1100
- 產品規格
- 96T
- 價格
- 2400.00
- 來源
- -
- 交叉反應
- Rat
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed
away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-2 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-rat IL-2 antibody
2. Lyophilized rat IL-2 standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-rat IL-2 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 31.2 pg/ml-2000 pg/ml
- 檢測樣品
- For quantitative detection of IL-2 in rat serum, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月, -20℃一年
- 背景資料
- Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. It can act as a growth hormone for both B and T lymphocytes. IL-2 has a well documented role in induction of pruritus, furthermore, it has been found to be higher in pruritic lesions of psoriasis compared to non-pruritic ones.
- 參考資料
- 1. Lowenthal, J. W., Zubler, R. H., Nabholz, M., MacDonald, H. R. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985. 2. Taniguchi T, Matsui H, Fujita T, Takaoka C, Kashima N, Yoshimoto R, Hamuro J (1983). "Structure and expression of a cloned cDNA for human interleukin-2". Nature 302 (5906): 305–310. 3. Fallahzadeh MK, Roozbeh J, Geramizadeh B , Namazi MR. Interleukin-2 serum levels are elevated in patients with uremic pruritus: a novel finding with practical implications. Nephrol Dial Transplant 2011.
