產品名稱：IL-1β Human ELISA Kit
- 產品編號
- BEK1094
- 產品規格
- 96T
- 價格
- 2400.00
- 來源
- -
- 交叉反應
- Human
- 免 疫 原
- -
- 產品簡介
- This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-IL-1β antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-IL-1β antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1β amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1β can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-human IL-1β antibody
2. Standard: 0.5ml (135 pg /mL)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-human IL-1β antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 1.56 pg/ml-100 pg/ml
- 檢測樣品
- For quantitative detection of IL-1β in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月
- 背景資料
- Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Pathak et al. (2011) proposed that IL1B blockade may be a viable therapy for patients with autoimmune inner ear disease or sensorineural hearing loss that fail to respond to corticosteroids.
- 參考資料
- 1. Pathak, S., Goldofsky, E., Vivas, E. X., Bonagura, V. R., Vambutas, A. IL-1-beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease. J. Immun. 186: 1870-1879, 2011. 2. del Rey, A., Roggero, E., Randolf, A., Mahuad, C., McCann, S., Rettori, V., Besedovsky, H. O. IL-1 resets glucose homeostasis at central levels. Proc. Nat. Acad. Sci. 103: 16039-16044, 2006.
