產品名稱：GDNF Rat ELISA Kit
- 產品編號
- BEK1070
- 產品規格
- 96T
- 價格
- 2400.00
- 來源
- -
- 交叉反應
- Rat
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GDNF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GDNF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDNF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDNF can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-Rat GDNF antibody
2. Lyophilized Rat GDNF standards: 2 tubes (10 ng/tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Rat GDNF antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 31.2 pg/ml-2000 pg/ml
- 檢測樣品
- For quantitative detection of GDNF in rat serum, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月, -20℃一年
- 背景資料
- Glial cell-derived neurotrophic factor, also known as GDNF, is a founding member of the GDNF family of ligands (GFL). In Rats, it is encoded by the GDNF gene. This gene mapped to 5p13.3-p13.1. Mutations in this gene may be associated with Hirschsprung's disease. GDNF is a small protein that potently promotes the survival of many types of neurons. The most prominent feature of GDNF is its ability to support the survival of dopaminergic and motorneurons. It promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. It also regulates kidney development and spermatogenesis, and it affects alcohol consumption.
- 參考資料
- 1. Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F (May 1993). "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons". Science 260 (5111): 1130–2. 2. Bermingham, N., Hillermann, R., Gilmour, F., Martin, J. E., Fisher, E. M. Rat glial cell line-derived neurotrophic factor (GDNF) maps to chromosome 5. Hum. Genet. 96: 671-673, 1995.
