產品名稱：G-CSF Mouse ELISA Kit
- 產品編號
- BEK1067
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Mouse
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-G-CSF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-G-CSF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the G-CSF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of G-CSF can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-mouse G-CSF antibody
2. Lyophilized Mouse G-CSF standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Mouse G-CSF antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 31.2 pg/ml-2000 pg/ml
- 檢測樣品
- For quantitative detection of G-CSF in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月, -20℃一年
- 背景資料
- Granulocyte colony-stimulating factor (G-CSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. The gene for G-CSF is located on chromosome 17, locus q11.2-q12, it has 4 introns, and that 2 different polypeptides are synthesized from the same gene by differential splicing of mRNA. G-CSF is a potent inducer of HSCs mobilization from the bone marrow into the bloodstream, although it has been shown that it does not directly affect the hematopoietic progenitors that are mobilized. G-CSF is also used to increase the number of hematopoietic stem cells in the blood of the donor before collection by leukapheresis for use in hematopoietic stem cell transplantation. It may also be given to the receiver, to compensate for conditioning regimens.
- 參考資料
- 1. Nagata S, Tsuchiya M, Asano S, Kaziro Y, Yamazaki T, Yamamoto O, Hirata Y, Kubota N, Oheda M, Nomura H (1986). "Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor". Nature 319 (6052): 415–8. 2. Thomas J, Liu F, Link DC (May 2002). "Mechanisms of mobilization of hematopoietic progenitors with granulocyte colony-stimulating factor". Curr. Opin. Hematol. 9 (3): 183–9.
